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Restriction Enzyme

Make a chart ...

Question

Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.

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Solution

The name of the restriction enzyme is Bam H 1.

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Q. Make a chart(with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.

Q. The DNA sequence that the restriction enzyme recognizes and the restriction site at which it cuts are always the same.

Q. Read the following statements and select the correct ones.
(i) Same kind of sticky ends are produced when a DNA is cut by different restriction enzymes.
(ii) Exonucleases make cuts at specific positions within the DNA.
(iii) Hind II was the first restriction endonuclease to be isolated.
(iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA.
(v) Presence of more than one recognition site for the same restriction enzyme within the vector complicates gene cloning.

Q. Read the following statements and select the correct ones.

(i) The same kind of sticky ends are produced when DNA has been cut by different restriction enzymes.

(ii) Exonucleases make cuts at specific positions within the DNA.

(iii) Hind II was the first restriction endonuclease to be isolated.

(iv) A bacteriophage can replicate within bacterial cells by integrating its DNA with bacterial DNA.

(v) The presence of more than one recognition site for an enzyme within the vector complicates the gene cloning.

Q. How many of the following statements are correct?
I. If a linear DNA molecule is digested with a restriction enzyme at four recognition sites, 4 fragments would be produced.
II. If a closed circular DNA molecule is digested with a restriction enzyme at six recognition sites, 6 fragments would be produced.
III. If a linear DNA molecule is digested with a restriction enzyme at four recognition sites on the DNA, 5 fragments would be produced.
IV. If a closed circular DNA molecule is digested with a restriction enzyme at six recognition sites, 7 fragments would be produced.

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Restriction Enzyme

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