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Restriction Enzyme

Make a chartw...

Question

Make a chart(with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.

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Solution

The name of the restriction enzyme is Bam H1.
The substrate for BamH1 enzyme is GGATCC. BAM H1 enzyme can make cut or nick on the G nitrogen base of restriction site and produces cut with sticky ends.

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Q. The DNA sequence that the restriction enzyme recognizes and the restriction site at which it cuts are always the same.

Q. Read the following statements and select the correct ones.
(i) Same kind of sticky ends are produced when a DNA is cut by different restriction enzymes.
(ii) Exonucleases make cuts at specific positions within the DNA.
(iii) Hind II was the first restriction endonuclease to be isolated.
(iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA.
(v) Presence of more than one recognition site for the same restriction enzyme within the vector complicates gene cloning.

Q.
(a)EcoRI is a restriction endonuclease. How is it named so? Explain (b) Write the sequence of DNA bases that the enzyme recognises. Mention the point at which the enzyme makes a cut in the DNA segment.

Q. Assertion :Restriction endonuclease recognize short palindromic sequence and cut at specific sites. Reason: When a restriction endonuclease acts on palindrome, it cleaves both the strands of DNA molecule.

Q. Read the following statements and select the correct ones.

(i) The same kind of sticky ends are produced when DNA has been cut by different restriction enzymes.

(ii) Exonucleases make cuts at specific positions within the DNA.

(iii) Hind II was the first restriction endonuclease to be isolated.

(iv) A bacteriophage can replicate within bacterial cells by integrating its DNA with bacterial DNA.

(v) The presence of more than one recognition site for an enzyme within the vector complicates the gene cloning.

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Restriction Enzyme

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