Question

Manipulation of DNA in genetic engineering became possible due to the discovery of : -

• A. Restriction endonuclease
• B. DNA ligase
• C. Transcriptase
• D. Primase

Answer 1

The correct option is A Restriction endonuclease

• Manipulation of DNA in genetic engineering became possible because of the discovery of restriction endonuclease.
• These are isolated from bacterial cells and are tools for molecular biologists,
• Several hundred restriction enzymes are now known, each with a specific sequence requirement dictating where it will cut DNA therefore, digesting DNA with a restriction enzyme creates a characteristic set of fragments, which can be isolated by electrophoresis and subsequently analyzed.

So, the correct answer is 'Restriction endonuclease'.