Mast cells contain vesicles that store large amounts of histamine. After staining with eosin, these vesicles are stained red in color. Identify which of the following interactions is involved between histamine and eosin?
Mast cells contain vesicles that store large amounts of histamine. After staining with eosin, these vesicles are stained red in color. Identify which of the following interactions is involved between histamine and eosin?
The correct option is B Electrostatic interaction
Eosin is the most common dye to stain the cytoplasm in histology. It is an acidic dye that binds to basic components of a cell, mainly proteins located in the cytoplasm. It gives a bright pink color that contrasts the dark blue nuclear hematoxylin staining Arginine, histidine, and lysine (at both acidic and neutral pH) and tryptophan (at acidic pH) residues of a protein bind electrostatically to carboxylic and phenolic groups of the dye to produce a stable water-soluble protein-dye complex. As Histamine is protein, Eosin and Histamine bind due to electrostatic interaction and gives red color.
So, the correct answer is “Electrostatic interaction”.
Eosin is the most common dye to stain the cytoplasm in histology. It is an acidic dye that binds to basic components of a cell, mainly proteins located in the cytoplasm. It gives a bright pink color that contrasts the dark blue nuclear hematoxylin staining Arginine, histidine, and lysine (at both acidic and neutral pH) and tryptophan (at acidic pH) residues of a protein bind electrostatically to carboxylic and phenolic groups of the dye to produce a stable water-soluble protein-dye complex. As Histamine is protein, Eosin and Histamine bind due to electrostatic interaction and gives red color.
So, the correct answer is “Electrostatic interaction”.
