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Question

Match column I and column II and choose the correct option.

Column-I Column-II
a. cryIAc (i) A. tumefaciens
b. Taq polymerase (ii) Thermus aquaticus
c. Native plasmid of S. typhimurium (iii) Specific Bt toxin gene
d. Natural genetic engineer for plants (iv) First recombinant DNA

A
a - iii, b - iv, c- ii, d - i
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B
a - iii, b - ii, c - iv, d - i
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C
a - i, b - ii, c - iii, d - iv
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D
a - ii, b - iii, c - iv, d - i
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Solution

The correct option is B a - iii, b - ii, c - iv, d - i
Bt toxin is encoded by cry gene.
Taq polymerase, a heat stable enzyme is isolated from bacterium Thermus aquaticus. This DNA polymerase is used in PCR.
Agrobacterium tumefaciens is considered as natural genetic engineer for plants. Cohen and Boyer accomplished the construction of first recombinant DNA by using native plasmid of S. typhimurium.

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