Match column I (enzyme) with column II (characteristic/ activity) and select the correct answer from the given

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Solution

- Taq DNA polymerase is the most common enzyme used for PCR amplification. This enzyme is extremely heat resistant with a half-life of 40 minutes at 95°C. At its optimal temperature (72°C), nucleotides are incorporated at a rate of 2–4 kilobases per minute. So, it is stable above 90 degree C.
- Exonucleases comprise a large set of structurally and biochemically characterized enzymes with diverse properties. Systematic characterization of exonucleases from the bacterium E. coli has provided much of our knowledge of this class of enzymes. Certain families of related exonucleases are found widely throughout bacteria, archaea, and eukaryotes, indicating the early evolution of nucleases and their important role in all cells. It helps to cleave the ends of linear DNA.
- Proteases have also found more specialized applications in processes for purification of non-protein products from animal or plant extracts including extraction of carbohydrate gums and muco-polysaccharides. These are used to degrade of protein structures.
- Chitinases are present in plants alongside various pathogenesis-related proteins as a part of plant defence mechanisms. It helps to brake the cell wall of fungus.
So, the correct option is A-3 B-1 C-5 D-2

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