Question

Match column I with column II, and choose the correct
combination from the options given below.

	Column I		Column II
a.	Bt toxin	1.	Meloidogyne incognita
b.	RNAi	2.	Insecticide
c.	Gene therapy	3.	Routine test for AIDS
d.	PCR	4.	ADA deficiency

• A. a-2, b-1, c-4, d-3.
• B. a-2, b-1, c-3, d-4.
• C. a-1, b-2, c-4, d-3.
• D. a-1, b-2, c-3, d-4.

Answer 1

The correct option is A a-2, b-1, c-4, d-3.

Bt toxin - Insecticide
Bt toxin is the most popular bio-pesticide. It is a crystal protein having insecticidal properties produced by the bacterium, Bacillus thuringiensis.

RNAi - Meloidogyne incognita
RNA interference (RNAi) or silencing of RNA is a method used to prevent the expression of a gene.

• Meloidogyne incognita is a nematode that infects roots of tobacco plants that grow into large galls or knots, damaging the crop and reducing the yield.
• To prevent the infection of tobacco plants from M. incognita, the tobacco plants are engineered such that nematode-specific genes are silenced using RNAi.

Gene therapy - ADA deficiency
Gene therapy is a process used to replace a defective gene with a correct one.
Gene therapy is used to cure Adenosine deaminase (ADA) deficiency caused by a mutation in the gene encoding ADA. It is accomplished by periodic infusion of genetically engineered lymphocytes with functional ADA cDNA.

PCR - Routine test for AIDS
Polymerase Chain Reaction is a technique used to amplify the gene of interest.
PCR technique can be used to detect AIDS in a patient even before the appearance of symptoms, by amplifying the nucleic acids of the HIV virus.

Final answer
b. a-2, b-1, c-4, d-3.