Question

Match column I with column II, and choose the correct
combination from the options given below.

	Column I		Column II
a.	ELISA	1.	Conventional method
b.	PCR	2.	Antigen antibody interaction
c.	r-DNA technology	3.	Molecular diagnosis
d.	Urine analysis	4.	Early detection of HIV

• A. a-4, b-3, c-2, d-1.
• B. a-1, b-2, c-3, d-4.
• C. a-2, b-4, c-1, d-3.
• D. a-2, b-4, c-3, d-1.

Answer 1

The correct option is D a-2, b-4, c-3, d-1.

ELISA - Antigen-antibody interaction
Enzyme-linked Immuno-sorbent Assay (ELISA) is used for the diagnosis of HIV/AIDS.
Antigen-antibody interaction and the specific identification of this complex is the main principle behind the ELISA technique.

PCR- Early detection of HIV
Polymerase Chain Reaction is a technique used to amplify the gene of interest.
PCR technique can be used to diagnose AIDS even before the appearance of symptoms, by amplifying the nucleic acids of the HIV virus.

rDNA technology - Molecular diagnosis
The techniques involved in manipulating genetic material such as DNA or RNA to produce recombinant DNA such as gene cloning and gene transfer are considered as rDNA technology.
Molecular diagnosis includes the popular methods designed using recombinant DNA technology. They are highly sensitive methods to detect HIV/AIDS, cancer, and other genetic mutations. It includes RFLP, VNTR based techniques.

Urine analysis - Conventional method
Urine analysis includes the detection of various disorders and urinary tract infections based on the composition, color, and concentration of analytes in urine.
It is a conventional method to detect disorders such as diabetes, jaundice, etc., and was majorly used before the invention of molecular diagnostic methods.

Final answer
b. a-2, b-4, c-3, d-1.