Question

Match column I with column II, and choose the correct combination from the options given below.

Column I	Column II
a. Hind II	1. Recognition sequence
b. pBR $322$	2. Restriction enzyme
c. GAATTC	3. Antibiotic resistance gene
d. $am{p}^R$	4. Cloning vector

• A. a - 3, b - 1, c - 4, d - 2
• B. a - 4, b - 3, c - 2, d - 1
• C. a - 2, b - 4, c - 1, d - 3
• D. a - 1, b - 2, c - 3, d - 4

Answer 1

The correct option is C a - 2, b - 4, c - 1, d - 3

$HindII$ - restriction enzyme
$HindII$ is the first restriction endonuclease that was isolated from Haemophilus influenzae, which recognises a specific sequence of 6 base pairs, $5$’- GTCGAC -$3$’and makes a cut in the DNA.

pBR$322$ - Cloning vector
pBR$322$ is a cloning vector that is used to insert a desired gene to form a recombinant DNA. This is inserted into a host cell such as Escherichia coli for cloning to form multiple copies.

GAATTC - recognition sequence
$5$’ - GAATTC - $3$’ is a recognition sequence for the restriction endonuclease EcoR I from $Escherichiacoli$ which cuts the DNA between G and A on both the strands producing sticky ends.

$am{p}^R$ - antibiotic gene
$am{p}^R$ is a gene sequence present on pBR $322$ responsible for the resistance against ampicillin antibiotics which can be used as a selectable marker.

Final answer:
c. a - 2, b - 4, c - 1, d - 3