Question

Match the columns I and II.

	List I		List II
A	Radioactive antibody	1	Substance that can be constructed in the laboratory. Its base sequence can be deduced from the amino acid sequence of the protein it codes for.
B	Artificial gene	2	Substance that can be used to identify colonies of genetically engineered bacteria that make a particular gene product.
C	Amplification	3	The use of entire array of genes of an organism in order to obtain the particular gene product.
D	To produce clone	4	Abnormal enhanced replication of a plasmid in each cell.
E	Shotgun cloning	5	A large population of identical cells.

• A. A- 2, B- 3, C- 4, D- 1, E- 5
• B. A- 2, B- 1, C- 4, D- 5, E- 3
• C. A-1, B- 4, C- 2, D- 5, E- 3
• D. A- 2, B- 3, C- 1, D- 4, E- 5

Answer 1

The correct option is B A- 2, B- 1, C- 4, D- 5, E- 3

• A radioactive antibody is a substance labeled with radioactive material that can be used to identify colonies of genetically engineered bacteria that make a particular gene product.
• The artificial gene is a segment of DNA that can be constructed in the laboratory. Its base sequence can be deduced from the amino acid sequence of the protein it codes for.
• Amplification is the process of abnormally enhanced replication of a plasmid in each cell to increase the number of copies of a particular DNA segment from one copy to a thousand copies.
• Clone is a large population of identical cells naturally derived from the common parent cell.
• Shotgun cloning or shotgun sequencing is the method for sequencing long DNA strands It is the use of the entire array of genes of an organism to obtain the particular gene product.

Thus, the correct answer is option B.