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Question

Match the enzyme in column I with its function in column II and choose the correct option.

Column IColumn II
A. β-galactosidase1. Joining of DNA fragments
B. Permease2. Peptide bond formation
C. Ligase3. Hydrolysis of lactose
D. Ribozyme4. Increases permeability to β-galactosidase

A
2,1,4,3
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B
3,4,1,2
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C
2,4,1,3
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D
1,2,4,3
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Solution

The correct option is A 3,4,1,2
β-galactosidase which is formed from gene lac Z brings about the hydrolysis of lactose, a disaccharide to form glucose and galactose. Permease is a protein which is formed from gene lacY enables transport of β-galactosidase into the cell by increasing its permeability. Ligase is an enzyme that facilitates the joining of DNA strands together by catalysing the formation of a phosphodiester bonds. Ribozymes are the RNA molecules which have the enzyme activity. Peptide bond formation during translation is affected by ribozyme.
Thus, the correct answer is option B.

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