Question

Match the following:

A	B
1. Sucrose	a. Micronutrient
2. Thiamine	b. Carbon source
3. Manganese	c. Macronutrient
4. Calcium	d. Vitamin

• A. 1 - b, 2 - a, 3 - d, 4 - c
• B. 1 - b, 2 - d, 3 - a, 4 - c
• C. 1 - c, 2 - d, 3 - c, 4 - a
• D. 1 - d, 2 - c, 3 - a, 4 - b

Answer 1

The correct option is B 1 - b, 2 - d, 3 - a, 4 - c

The given table represents the ingredients that are added to a tissue culture medium in column A and the nutrient category that they belong to in column B.
Tissue culture involves growing specific plant parts, cells or tissues, known as explants, in a sterile artificial medium under controlled conditions. The culture medium used for this purpose contains all essential nutrients and growth hormones that would be necessary for the multiplication and differentiation of the cells of the explant to finally form a plantlet. The proportions of the ingredients vary based on the explant and the purpose of the culture.
An ideal culture medium should have

• inorganic salts as sources of micronutrients (manganese, iron, zinc, copper, boron, molybdenum) and macronutrients (nitrogen, sulphur, phosphorus, potassium, magnesium, calcium).
• source of carbon and energy - sugars such as sucrose, glucose, fructose, etc
• nitrogen sources for building up proteins - amino acids
• vitamins such as thiamine, vitamin $B_3$
• growth hormones such as auxins, gibberellins and cytokinins, etc are added to induce growth, development and morphological changes in the explant. Their concentrations vary depending on the purpose of the culture.

A	B
1. Sucrose	b. Carbon source
2. Thiamine	d. Vitamin
3. Manganese	a. Micronutrient
4. Calcium	c. Macronutrient