Match the following component of the electrophoresis unit in column I with its use in column II.
Column I
Column II
1. Comb
a. Prevents DNA from floating out of wells and makes the position of the DNA easier to see.
2. Gel box
b. Houses gel and expose it to an electrical current generated by a power supply.
3. Micropipette
c. Contains DNA molecules of known size that may be used to gauge the location of fragments of interest in the gel.
4. Loading buffer
d. Creates wells on one end of the gel for loading of the DNA
5. DNA standard size
e. Used to transfer small volumes of liquid.
Match the following component of the electrophoresis unit in column I with its use in column II.
| Column I | Column II |
| 1. Comb | a. Prevents DNA from floating out of wells and makes the position of the DNA easier to see. |
| 2. Gel box | b. Houses gel and expose it to an electrical current generated by a power supply. |
| 3. Micropipette | c. Contains DNA molecules of known size that may be used to gauge the location of fragments of interest in the gel. |
| 4. Loading buffer | d. Creates wells on one end of the gel for loading of the DNA |
| 5. DNA standard size | e. Used to transfer small volumes of liquid. |
The correct option is B 1-d, 2 –b, 3-e, 4-a, 5-c
Plastic comb in the electrophoresis gel unit is used to create wells, while casting the gel is for loading the DNA.
Gel box is a rectangular unit used to hold TAE buffer and the gel and to provide a suitable platform for current flow and movement of DNA under the influence of the current.
Micropipette is a small compact laboratory instrument which is used to transfer a very small volume (micro level) of liquid sample for the analysis.
Loading the buffer gives weight to the DNA fragments thus not allowing the DNA sample to ooze out of the well.
Determining the size of DNA in electrophoresis is accomplished by comparison to size standards, also called markers or ladders. Typical size standards are made up of DNA fragments in variable length in the range of 10bp to 1000bp (base pair) increments.
Plastic comb in the electrophoresis gel unit is used to create wells, while casting the gel is for loading the DNA.
Gel box is a rectangular unit used to hold TAE buffer and the gel and to provide a suitable platform for current flow and movement of DNA under the influence of the current.
Micropipette is a small compact laboratory instrument which is used to transfer a very small volume (micro level) of liquid sample for the analysis.
Loading the buffer gives weight to the DNA fragments thus not allowing the DNA sample to ooze out of the well.
Determining the size of DNA in electrophoresis is accomplished by comparison to size standards, also called markers or ladders. Typical size standards are made up of DNA fragments in variable length in the range of 10bp to 1000bp (base pair) increments.
