Match the following enzymes of DNA replication with their respective functions:
p. DNA polymerasei. Helps unzip the DNA double helixq. DNA ligaseii. Seals nicks between Okazaki fragmentsr. DNA helicaseiii. Lays RNA primers to which polymerase can add nucleotidess. Primaseiv. Adds nucleotides to the free 3′ end of RNA primers
p - iv, q - ii, r - i, s - iii
Among the major enzymes involved in DNA replication, DNA polymerase primarily performs the task of adding nucleotides to the free 3’ end of the RNA primers. DNA ligase seals up any nicks formed in the newly synthesised DNA strands, such as those between Okazaki fragments in the lagging strand and between the fragment that replaced RNA primer and the rest of the nucleotides added by DNA polymerase. However, ligase does not add nucleotides (which is done only by polymerase) to fill in gaps. DNA helicase is involved in unzipping the DNA double helix, which it achieves by breaking up of the hydrogen bonds between the nucleotides of each base pair. Primase, specifically the RNA primase enzyme, lays down the short RNA primers at the beginning of replication so that, DNA polymerase can start adding nucleotides to the free 3’ end of the primers to form the new strands.