Match the following enzymes to their functions in replication of DNA.

Adding nucleotides

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Joining DNA strands

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Unwinds DNA

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RNA primer synthesis

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Solution

Enzymes involved in the replication of DNA.
DNA helicase - DNA helicase disrupts the hydrogen bonding between base pairs to separate the strands into a Y shape steructure known as the replication fork.
RNA primase - It synthesizes short RNA sequences called primers. These primers serve as a starting point for DNA synthesis.
DNA polymerase - They are responsible for creating the new strand by a process called elongation.
DNA ligase - It connects Okazaki fragments (small fragments formed during the replication of double-stranded DNA).

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Match the following enzymes of DNA replication with their respective functions:
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Match the following enzymes of DNA replication with their respective functions:

p. DNA polymerase i. Helps unzip the DNA double helix

q. DNA ligase ii. Seals nicks between Okazaki fragments

r. DNA helicase iii. Lays RNA primers to which polymerase can add nucleotides

s. Primase iv. Adds nucleotides to the free 3’ end of RNA primers

Q. Match the following enzymes of DNA replication with their respective functions:

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Q. Match the following enzymes to their functions in replication of DNA.

Q. Rearrange the following in the current sequences to accomplish an important biotechnological reaction.
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA

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