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Question

Match the following of column I with that of column II.

Column I Column II
i. cDNA p. Molecular diagnosis by DNA amplification
ii. Auto-radiography q. Radiolabelled probes
iii. PCR r. Reverse transciptase
iv. ADA s. Erythrocytes
t. Lymphocytes

A
i – q, ii – r, iii – p, iv – s
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B
i – r, ii – q, iii – p, iv - s
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C
i – r, ii – p, iii – q, iv - t
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D
i – r, ii – q, iii – p, iv – t
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Solution

The correct option is D i – r, ii – q, iii – p, iv – t

Polymerase chain reaction is the molecular process which amplifies a small section of a specific DNA sequence. It is used in molecular diagnostic procedure to amplify a small amount of genetic material so that the disorder can be identified.

Auto-radiography is the diagnosis technique which uses the principle of hybridisation between specific radiolabeled probe DNA sequences with the target sequence. Once these radioactive probes bind with the sequence it sends out signals to sensors.

Complementary DNA (cDNA) is DNA synthesized by the enzyme reverse transcriptase from a single-stranded RNA template. cDNA is often used to clone eukaryotic genes in prokaryotes.

Adenosine deaminase deficiency (ADA deficiency) is caused by deletion of the ADA gene. This gene is responsible for making ADA enzymes that are found especially in lymphocytes. Lymphocytes are an important part of the immune system and help protect the body from infections.


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