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Question
Match the following steps of PCR to their respective temperatures:
Match the following steps of PCR to their respective temperatures:
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Solution
PCR occurs in three steps:
Denaturation: The temperature is maintained at 94°C. The high temperature melts the hydrogen bonds and separates the two strands of DNA.
Anealing: It occurs at 55°C. The two oligonucleotide primers anneal with the separate strands.
Extension: It carries out at 72°C. Finally, DNA polymerase froms the complimentary strand.
Denaturation: The temperature is maintained at 94°C. The high temperature melts the hydrogen bonds and separates the two strands of DNA.
Anealing: It occurs at 55°C. The two oligonucleotide primers anneal with the separate strands.
Extension: It carries out at 72°C. Finally, DNA polymerase froms the complimentary strand.
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