Question

Match the lists and find the correct option:

$\begin{array}{cc}\text{I}& \text{II}\\ \text{(a) Colony hybridisation}& \text{1. Transfer of recombinant DNA into host cell}\\ \text{(b) Gel electrophoresis}& \text{2. Selection of cells containing the desired gene}\\ \text{(c) Gradient centrifugation}& \text{3. Separation of DNA fragments}\\ \text{(d) Polymerase chain reaction}& \text{4. Purification of DNA}\\ & \text{5. Gene cloning in thermocycler}\end{array}$

• A. a – 4, b –5, C –2, d –3
• B. a – 1, b – 2, c – 3, d –4
• C. a – 2, b –3, c – 4, d –5
• D. a –3, b –4, c –5, d –2

Answer 1

The correct option is C a – 2, b –3, c – 4, d –5

Selection of cells containing desired gene is done by colony hybridization. Gel electrophoresis is a technique used for separating DNA fragments. Centrifugation is done to purify DNA. PCR is used for amplification of gene, invitro inm a thermocyler.