Question

Match the organism with its use in biotechnology.

a	Bacillus thuringiensis	i	Cloning vector
b	Thermus aquaticus	ii	Construction of first rDNA molecule
c	Agrobacterium tumefaciens	iii	DNA polymerase
d	Salmonella typhimurium	iv	Cry proteins

Select the correct option from the following

• A. a - iii, b - ii, c - iv, d - i
• B. a - iii, b - iv, c - i, d - ii
• C. a - ii, b - iv, c - iii, d - i
• D. a - iv, b - iii, c - i, d - ii

Answer 1

The correct option is D a - iv, b - iii, c - i, d - ii

(a) Bacillus thuringiensis produces the cry proteins, which are Bt toxins and have insecticidal properties.
(b) The highly thermostable DNA polymerase from Thermus aquaticus is ideal for both manual and automated DNA sequencing because it is fast & highly processive and remain active during the high temperature induced denaturation of double stranded
DNA.
(c) The tumor-inducing (Ti) plasmid of Agrobacterium tumifaciens has been modified into a cloning vector. It is used to deliver required genes into plants.
(d) Salmonella typhimurium is the first recombinant DNA that cut the piece of DNA from a plasmid carrying antibiotic resistance gene in the bacterium and linked it to the plasmid of E.coli.