Question

Maximum application of animal cell culture technology today is in the production of

• A. Vaccines
• B. Edible protein
• C. Insulin
• D. Interferon

Answer 1

Answer: (A)

Vaccines

Animal cell culture is culture of extracted animal cells in aseptic laboratory with environmental conditions same as in vivo. Cultured animal cells are used in the production of vaccines producing viruses. Vaccines for polio, rabies, chicken pox, measles and hepatitis B are produced using animal cell culture. Insulin production includes insertion of chemically synthesized A and B chain DNA sequences into plasmids followed by transformation of E. coli with plasmid carrying the gene. Replication of recombinant plasmid in E. coli produces insulin. Interferon is a carbohydrate containing proteins that are produced by fibroblasts and leukocytes in response to immune stimuli. It has antiviral and anticancer properties and is produced by transformation of E. coli with recombinant plasmid carrying the gene for interferon. Edible proteins are produced by molecular farming, i.e., transfer of genes of proteins to plants and animals for production of proteins. Option A is the correct answer.