The correct option is B Segregation
The monohybrid phenotypic ratio of 3:1 was the important clue for Mendel to crack the law of segregation. When he crossed the two pea plants in a scientific manner, the result obtained were in the ratio of 3:1 in F2 generation and he concluded the law of segregation. The law states that during gamete formation, the factors or alleles of a pair segregate from each other such that a gamete receives only one of the two factors.