Question

Mention the steps involved in DNA fingerprinting

Answer 1

DNA Fingerprinting:

1. DNA fingerprinting is a technique for identifying and evaluating variations in DNA sequences based on polymorphism and variations.
2. Some instances of DNA fingerprinting applications are as follows: It's utilized to find criminal suspects, paternity tests, and to determine the evolutionary history of an organism.
3. Steps involved in DNA fingerprinting are:
   1. The first step is to isolate the DNA.
   2. DNA is broken down by restriction endonuclease enzymes.
   3. The digested particles are separated according to their size using electrophoresis.
   4. Blotting the individual bits onto synthetic membranes made of nylon.
   5. Hybridize the pieces using tagged VNTR probes.
   6. The hybrid components were examined using autoradiography.