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Question

Method in which recombinant DNA is directly injected into the nucleus of animals cell by using microneedles is called as

A
Gene gun method
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B
Biolistic method
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C
Microinjection method
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D
Indirect method
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Solution

The correct option is B Microinjection method
Microinjection technique involves delivering foreign DNA into a living cell (a cell, egg, oocyte, embryos of animals)using a glass micropipette. The tip of the pipette is about 0.5 mm diameter which resembles an injection needle. A powerful microscope will be used to deliver foreign DNA. Cells to be microinjected are placed in a container and holding pipette is placed in the microscopic view.
The holding pipette holds a target cell at the tip when gently sucked. The tip of the micropipette containing foreign DNA is then injected through the membrane of the cell to inject the contents. Once injected, the pipette will be taken out.

So, the correct answer is the 'Microinjection method'.

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