Question

Method of raising new plants in large number from a small plant tissue over a culture medium is

• A. Callus, formation
• B. Micropropagation/tissue culture
• C. Micrografting
• D. Juvenility

Answer 1

Answer: (B)

Micropropagation/tissue culture

Method of raising new plants in large number from a small plant tissue over a culture medium is micropropagation. The step of micropropagation includes:

1) The plant which needs to be propagated is chosen.

2) The plant part which has to be used as an explant is chosen. It can be the pollen, leaf, stem etc of the stock plant.

3) The explant is surface sterilized.

4) Culture the explant on a nutrient media.

5) After growth of root and shoot, it undergoes hardening treatment where it is grown under temperature available on fields to prepare them for growth.

6) In the final stage of plant micropropagation, the plantlets are removed from the plant media and transferred to the soil.