The correct option is A Escherichia coli and Agrobacterium tumefaciens
Escherichia coli and Agrobacterium tumefaciens are microbes found to be very useful in genetic engineering.
E.coli is a mobile, Gram-negative, rod-shaped bacterium which is a normal inhabitant of the human colon. It is most extensively used in bacterial genetics and molecular biology.
Agrobacterium tumefaciens is a soil bacterium. It has Ti plasmid (Tumour inducing plasmid) and it can be used for the transfer of the desired gene in dicot plants.