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B
Persist but remain thin-walled
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C
Degenerate before maturity
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D
Persist and become thickened
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Solution
The correct option is C Degenerate before maturity
The cells of middle layers are generally ephemeral i.e. they are short lived. They become flattened and get crushed during meiosis in the pollen mother cells (PMC). So, middle layers degenerate before maturity. However the exception is Lilium and Ranunculus where middle layers may persist.