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B
Having A=T and C=G
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C
Being highly twisted
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D
Lacking binding to histones
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Solution
The correct option is B Lacking binding to histones
Mitochondrial DNA differs from Nuclear DNA as it lacks histones. This is because, mitochondria as an organelle was an independent prokaryotic cell, that during evolution became a part of the eukaryotic cell (endosymbiotic theory), where it retained its circular DNA that does not contain histones instead, is kept together by a different type of protein analogous to histone.
So, the correct answer is 'Lacking binding to histones'