Question

Mona followed this procedure for staining the leaf peel

(i) She plucked the leaf of lily.

(ii) Cut the leaf into pieces.

(iii) Stained the leaf peel.

(iv) Removed the leaf peel.

• A. (i), (ii), (iii), (iv)
• B. (i), (ii), (iv), (iii)
• C. (iv), (iii), (ii), (i)
• D. (ii), (i), (iv), (iii)

Answer 1

The correct option is B

(i), (ii), (iv), (iii)

The correct option is B

Explanation for the correct option

• Leaf peel is a thin layer from the surface of a leaf.
• It is obtained to visualize the epidermal cells and stomata on a leaf.
• To perform the procedure, one should first collect the leaf, followed by cutting of leaf. The leaf peel is removed and stained to observe under the microscope in the next step.

Explanation for incorrect options

Option A:

• Leaf peel cannot be stained until it is removed from the leaf. Thus, this order is incorrect.

Option C:

• To obtain a leaf peel, a healthy leaf is required first. Thus, the following series of steps is incorrect.

Option D:

• To cut the leaf, it is first required to pluck the leaf. Thus, this is the incorrect order.

Final answer: (i), (ii), (iv), (iii) is the correct protocol to follow.