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Question

Most suitable method of introducing foriegn DNA into a plant cell is

A
Biolistics
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B
Microinjection
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C
Lipofection
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D
Heal shock method
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Solution

The correct option is B Biolistics
The most suitable method of introducing foreign DNA into a plant cell is biolistics. In biolistics, the impregnation of the cells with nucleic acids or with other biological molecules is achieved by coating the molecules that are intended to be introduced into the cells onto microcarriers. These microcarriers could be gold microparticles or High density tungsten microparticles. Then a gene gun is used to carry out the procedure. The gene gun with the help of helium pulse accelerates these particles to extremely high velocities. The result is they are able to penetrate the cell walls and membranes and get into the cell.

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