Mutations can be artificially induced by
Mutations can be induced by several methods. The three general approaches used to generate mutations are radiation, chemical and transposon insertion.
In addition to X-rays, other types of radiation treatments that have proven useful include gamma rays and fast neutron bombardment. These treatments can induce point mutations (changes in a single nucleotide) or deletions (loss of a chromosomal segment).
Chemical mutagens work mostly by inducing point mutations. Point mutations occur when a single base pair of a gene is changed. These changes are classified as transitions or transversions. Two major classes of chemical mutagens are routinely used. These are alkylating agents (such as ethyl methane sulphonate (EMS), ethyl ethane sulphonate (EES)) and base analogs (5-bromouracil and 2-aminopurine).
Therefore, the correct answer is option C.