Question

Name a few enzymes involved in DNA replication other than DNA polymerase and ligase. Name the key functions for each of them.

Answer 1

DNA replication:

Replication is the process by which a DNA produces it’s identical copies.


Enzymes involved in DNA replication:

Helicase, primase, polymerase and ligase are some of the enzymes involved in DNA replication.


Helicase:

Helicase breaks the hydrogen bonds in between the two strands of the DNA and helps in the unwinding of the DNA.

Primase:

Primase is the enzyme that synthesizes RNA primer. DNA polymerase cannot start DNA synthesis de novo, it only adds dNTPs to the RNA primer created.


Polymerase :

DNA dependent: DNA polymerase is the enzyme involved in the replication of DNA, because it adds new nucleotides on the 3'-OH position of the previous nucleotide by a nucleophilic attack.


Ligase:

Ligase helps in the joining of the Okazaki fragments together by catalyzing the formation of phosphodiester bonds.