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Question

Name the factor which will not affect the enzyme activity with a limited amount of chemical substrate.

A
pH level
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B
Inhibitors
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C
Temperature
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D
Enzyme cofactors
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E
Enzyme concentration
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Solution

The correct option is E Enzyme concentration
If the chemical substrate is present in limited quantity, enzyme concentration would not affect the rate of reaction as substrate won't be available to occupy the enzyme active sites. Hence increasing the enzyme concentration would not increase the reaction rate if substrate concentration is a limiting factor. All other factor mentioned affect the reaction rate independent of substrate concentration.
So, the correct answer is option E.

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