Question

Name the selectable markers in the cloning vector pBR322?

Answer 1

The cloning vector requires a, origin of replication, selectable markers, a cloning site and/or a reporter gene. The Cloning vector allows for a site for DNA to be inserted into the plasmid.
The origin of replication allows the plasmid to replicate. pBR322 is a medium replication plasmid, which means it has 10-100 copies per cell. The selectable markers allow the cell that the plasmid is in, be selected for if it does or does not have the plasmid. The cloning site is a site that is where the new genes are inserted into the plasmid. The reporter gene is another way of testing if the cell picked up the modified plasmid or not. This is done by having all of the cells with the plasmid create a product to identify it form the other cells.
This plasmid pBR322 is very useful because of the selectable markers in it. It has two selecgtable markers, a resistance to Ampicillin and Tetracycline. This allows the cells with the plasmid
to be selected for. without the selectable marker, all cells would grow, not just the cells that the cells that contain the desired genes. Vector requires a selectable marker, which helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.