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Question

Name the technique that is used for separating the fragments of DNA cut by restriction endonucleases.

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Solution

The technique is called Gel Electrophoresis. DNA is negatively charged, forced to move towards the anode, electric field agarose gel matrix, separated according to their size/sieving effect, smaller fragments move faster and further than the larger. They can be separated by forcing them to move towards the anode under an electric field through a medium/matrix.

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