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B
Competition and mutation
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C
Isolation and competition
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D
Isolation and variation
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Solution
The correct option is A Isolation and mutation
Accumulated random changes within separate isolated populations due to genetic drift are not shared and eventually, lead to incompatible reproductive differences between them thus leading to new species.
Mutation is a permanent alteration of the nucleotide sequence of the genome of an organism or extrachromosomal DNA or other genetic elements which lead to new species
So, the correct answer is 'Isolation and mutation'