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B
Rhizobium
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C
Azotobacter
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D
Azolla
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Solution
The correct option is C Azotobacter Non-symbiotic nitrogen fixation is carried out by free living organisms in the soil, such as bacteria and bluegreen algae. Bacteria include aerobic bacteria such as Azotobacter and anaerobic bacteria, such as Clostridium, Chlorobium and Chromatium.
Blue green algae include Chroococcus, Rivularia, Anabaena, Tolypothrix and Nostoc. These organisms contain an enzyme system called as Nitrogenase, which is a Mo-Fe (Molybdenum-ferredoxin) protein. This progressively reduces the dinitrogen molecules to form ammonia, with the help of ferredoxin and energy from ATP.