The correct option is D All of these.
Answer is option D i.e. "All of these"
The nucleolus is a round body located inside the nucleus of a eukaryote. It is not surrounded by a membrane. Granular zone is in the peripheral region of the nucleolus, which is surrounded by the associated chromatin. Fibrillar zone contains thread-like fibers that contains ribonucleoprotein. The amorphous component of the nucleolus begin to disappear during the prophase stage.