The correct option is C Secondary constriction
The nucleolus is formed by the secondary constriction. The nucleolus is the largest structure present in the nucleus of eukaryotic cells where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like the assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions.