Question

Observe the given figure and answer the questions given below.

Which fraction would show the highest rate of oxygen consumption? Give a reason for your answer.

Answer 1

The fraction C is centrifuged at the speed of 15,000 g. The mitochondria can be separated in the fraction by centrifuging at the speed of 10,000 - 20,000 g for 15 - 20 minutes. The mitochondria is the organelle where the cellular respiration takes place. The oxygen is taken by the cell which is used by the mitochondria for respiration. The oxygen is used for the break down of Acetyl coA (a 2 carbon molecule formed from glucose) which is used for the production of ATP. The cytrochrome complexes which are involved in the electron transport chain helps in the formation of ATP. The ATP is the source of energy required for carrying the other metabolic reactions. So, the highest rate of consumption of oxygen will be seen in the fraction C.