Question

Okazaki fragments are joined in the correct sequence by:

• A. DNA polymerase
• B. DNA ligase
• C. RNA polymerase
• D. Primase

Answer 1

The correct option is B DNA ligase

DNA ligase is a specific type of enzyme, a ligase, that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. Hence, it is called as molecular glue.
The formation of new chain by DNA polymerase during replication always takes place in 5' - 3' direction. As a result of this, one chain of DNA is continuously formed and it is termed as leading strand. While the other chain is discontinuous and is made up of small segments called Okazaki fragments. This discontinuous chain is termed as lagging strand. Ultimately, the Okazaki fragments are joined together by an enzyme DNA ligase.