Question

One of the following is a wrong match.

• A. Micropropagation - In vitro production of plants in large numbers
• B. Callus - Unorganised mass of cells produced in tissue culture
• C. Vector DNA - Site for tRNA synthesis
• D. Somatic hybridization - Fusion of two diverse cells

Answer 1

Answer: (C)

Vector DNA - Site for tRNA synthesis

Micropropagation is a plant tissue culture technique in which a small plant part is artificially cultured to produce new plants. An organised cell mass produced in the process of plant tissue culture is called as callus. From callus, roots and shoots can be differentiated based upon the ratio of phytohormones- auxin and cytokinin. Somatic hybridization is a plant breeding technique in which two somatic cells are artificially fused after removing the cell wall. Some chemicals like polyethylene glycol (PEG) help the cell protoplasm to fuse. Vector DNA is the DNA in which we can insert the gene of choice to generate multiple copies. A good vector should be easy to multiply and should have easy selection mechanism so that the vector having foreign gene insert can be segregated from the vector not having foreign gene insert. Plasmids and bacteriophages are commonly used natural vectors. A large number of artificially produced vectors (example: pBR322, phagemids, cosmids, etc.) are available for cloning of genes. Thus, the correct answer is option C.