1
Question
Ori Site: Initiation of replication :: Antibiotic resistance gene: __________
Ori Site: Initiation of replication :: Antibiotic resistance gene: __________
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Solution
The correct option is B Selectable marker
A DNA sequence carrying the gene of interest into the host cell is called a vector. The vector is used in creating copies of gene of interest. Thus its called cloning vectors. The cloning vector with the gene of interest inserted in it is called recombinant DNA.
This recombinant DNA is then inserted into the host cell. The selectable markers present in the cloning vectors helps in the detection of transformed cells and eliminates the non transformed host cells. Antibiotic resistance gene is an example of selectable marker.
Generally, genes that provide resistance to antibiotics such as ampicillin, tetracycline, chloramphenicol or kanamycin are used as selectable markers.
The Ori sequence present in the cloning vector helps in initiation of replication. This is a specific sequence of nucleotides that is recognised by the helicase enzymes during replication of DNA.
The recognition sites are specific palindromic sequences where the restriction enzymes make their cuts in the cloning vector. Palindromic sequences are specific sequences within the DNA that read the same whether read from 5’ -> 3’ or from 3’ -> 5’direction. The restriction enzymes can recognise these sequences and make cuts at specific locations of these palindromic sequences.
The repressor gene is often found in certain operons and the products of this gene, generally, represses the expression of the operon.
The promoter is a sequence that is located just upstream of the initiation codon of a structural gene. This is the site at which the RNA polymerase enzyme binds to initiate the process of transcription of the gene into an mRNA.
A DNA sequence carrying the gene of interest into the host cell is called a vector. The vector is used in creating copies of gene of interest. Thus its called cloning vectors. The cloning vector with the gene of interest inserted in it is called recombinant DNA.
This recombinant DNA is then inserted into the host cell. The selectable markers present in the cloning vectors helps in the detection of transformed cells and eliminates the non transformed host cells. Antibiotic resistance gene is an example of selectable marker.
Generally, genes that provide resistance to antibiotics such as ampicillin, tetracycline, chloramphenicol or kanamycin are used as selectable markers.
The Ori sequence present in the cloning vector helps in initiation of replication. This is a specific sequence of nucleotides that is recognised by the helicase enzymes during replication of DNA.
The recognition sites are specific palindromic sequences where the restriction enzymes make their cuts in the cloning vector. Palindromic sequences are specific sequences within the DNA that read the same whether read from 5’ -> 3’ or from 3’ -> 5’direction. The restriction enzymes can recognise these sequences and make cuts at specific locations of these palindromic sequences.
The repressor gene is often found in certain operons and the products of this gene, generally, represses the expression of the operon.
The promoter is a sequence that is located just upstream of the initiation codon of a structural gene. This is the site at which the RNA polymerase enzyme binds to initiate the process of transcription of the gene into an mRNA.
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