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B
Electron microscopy
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C
Phase contrast microscopy
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D
X-ray diffraction
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Solution
The correct option is B Electron microscopy Osmium tetroxide is used in electron microscopy. The compound can be used as a fixative and a heavy metal stain. Osmium tetraoxide is a good fixative. They serve as an excellent stain for lipids in membranous structures and vesicles. They help in fixation and preservation of the lipids in the cellular membranes.