Question

Difference between gene cloning and recombinant DNA technology

Answer 1

Gene cloning is an artificial process of producing exact copies of a particular gene using genetic engineering techniques.

There are two types of gene cloning:

1. In vivo gene cloning : In this type the copies of genes are produced inside a host cell using restriction endonuclease and ligase enzymes.

2. In vitro gene cloning : In this type the copies of genes are produced in a reaction tube using the polymerase chain reaction.

Recombinant DNA technology is a technology that works by taking DNA from two different sources and combining that DNA into a single molecule. This recombinant DNA is then inserted into a desired organism to produce hybrid offsprings.