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Gene Therapy

how is PCR us...

Question

how is PCR used to detect gene mutations in case of suspected cancer patients?

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Solution

PCR can amplify a specific DNA fragment with mutation that can be than detected on the gel with electrophoresis.

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Q. X technique is now routinely used to detect HIV in suspected AIDs patients. It is being used to detect mutations in genes in suspected cancer patients too. It is a powerful technique to identify many other genetic disorders. Identify X-

Q. Assertion :PCR can detect very low amounts of DNA and is now routinely used to defect HIV in suspected AIDS patient.
Reason: PCR is a powerful technique to identify many other genetic disorders.

Q. Which technique do you suggest to detect suspected cancer patients?

Q. A certain patient is suspected to be suffering from AIDS.Which diagnostic technique will you recommend for its detection?

Q. Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentry DNA in a clone of cells,followed by its detection using autroradiography because:

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