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Selection of Transformed Cell

State what ha...

Question

State what happens when a gene is inserted in Sal I site of pBR322 plasmid.

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Solution

Sal 1 site in the plasmid confers resistance to tetracycline to the vector. If any foreign gene is inserted at this site, the resistance to this antibiotic will be hampered.

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Q. If gene of interest was inserted at Sal l site in pBR

322

the resulting plasmid will confer resistance to?

Q. Plasmid pBR322 has Pst I restriction enzyme site within gene amp^Rthat confers ampicillin resistance. If this enzyme is used for inserting a gene for ß-galactoside production and the recombinant plasmid is inserted in an E. coli strain

Q. The gene of interest (GOI) is inserted at the
BamH I site in pBR322. The resulting plasmid is introduced into bacteria. The bacteria will have resistance against which of the following antibiotics?

p B R 322 a m p^{R}

t e t^{R}

genes are present.When we ligate a foreign DNA at

P v u I

site, then recombiant plasmids will lose the resistance to the ____________.

Q. Which among the following are found in

p B R 322

(1)

1

(2)

Ampicillin resistance site

(3)

Tetracycline resistance site

(4)

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Selection of Transformed Cell

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