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Ligase

What is meant...

Question

What is meant by "tet^R gene has Bam HI recognition site".?

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Solution

A recognition site is a location on a nucleic acid (DNA) at which the restriction enzyme makes cut so that gene of interest can be inserted there. Tet ^R gene has Bam HI recognition site means that the gene sequence which provides tetracycline resistance in plasmid vector pBR32 is a recognition site for the enzyme Bam HI. In otherwords Bam HI recognises the sequence at which tetracycline resistance gene (Tet ^R) is present to make a cut.

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Q. In pBR322, tetracycline resistance gene (

t e t^{R}

) has recognition site for which of the following restriction endonuclease?

Q. Assertion :Recognition site should be preferably single and responsive to commonly used restriction enzymes. Reason: In

p B R 322

D N A

is ligated generally in the area of

B a m - H I

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Q. Assertion :Recognition site should be preferably single and responsive to commonly used restriction enzyme. Reason: In pBR322 alien DNA is ligated generally in the area of Bam HI site of tetracyline resistance gene.

Q. Assertion :The predominant site for control of gene expression in prokaryotes is transcription initiation. Reason: The activity of RNA polymerase is regulated by accessory proteins, which affect recognition of start sites.

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