Question

What is primers? Mention its role and the number of primers required in each cycle of PCR. Give the characteristic feature and source organism of the DNA polymerase used in PCR.

Answer 1

Primers are small chemically synthesised oligonucleotides that are complementary to the regions of DNA.
No. of primers required=2
Primers are used to bind with sequence on the either side of the DNA.
The enzyme Taq DNA polymerase is obtained from bacteria Thermus aquaticus. Taq DNA polymerase enzyme works on very high temperature.