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Applications of Biotechnology

Palindromic s...

Question

Palindromic sequences in DNA

A

form "blunt" ends when cut by restriction enzymes

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B

reflect the same sequence on two sides.

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C

are not useful in recombinant DNA experiments.

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D

all of these

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Solution

The correct option is B
reflect the same sequence on two sides.

A palindromic sequence is a sequence made up of nucleic acids within double helix of DNA and/or RNA that is the same when read from 5’ to 3’ on one strand and 3’ to 5’ on the other, complementary, strand.

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Similar questions

Q. Assertion: Restriction enzymes recognise specific palindromic sequences.
Reason: Palindromic sequences are DNA sequences that are read the same way when the orientation is kept the same in both the strands.

Q. Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at 'specific-recognition sequence'. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?

Q. Which one of palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme ?

Q. Assertion: Sticky ends forms hydrogen bonds with their complementary cut counterparts.
Reason: In recombinant DNA technology, sticky end producing restriction enzymes are preferred over blunt ends producing restriction enzymes.

Q. Assertion :Restriction enzymes recognise palindromic sequences. Reason: Palindromic sequences read same in both directions of the two strands.

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