Question

PCR technique is used in

• A. Production of transgenic microbes
• B. Production of genetically modified food
• C. Forensic investigation
• D. Gene transfer

Answer 1

The correct option is C Forensic investigation

PCR is a technique used to amplify the gene of interest in three steps namely denaturation of target DNA (thermal cycle to separate the DNA strands), annealing of primers to the ssDNA and polymerization (extension of primer into complete DNA strand complementary to the template strand). After completion of one cycle of PCR, two copies of the target DNA are produced both of which serve as template for next PCR cycle. Thus, a small sample collected from crime site is amplified and is used to compare with DNA of suspect using radioactive probes.

So, the correct answer is option C.